Download e-book for iPad: A Natural History of Trees: of Eastern and Central North by Donald Peattie, Paul Landacre, Robert Finch

By Donald Peattie, Paul Landacre, Robert Finch

One of 2 actual classics of yankee nature writing now in paperback; the opposite is A typical heritage of Western Trees.

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Extra info for A Natural History of Trees: of Eastern and Central North America

Sample text

Rhizogenestransformed roots of three forage legumes (Trifolium pratense, Macroptilium atropurpureum, and Medicago sativa) were inoculated with Rhizobium. It was suggested by the authors that the inhibition of nodulation was linked to the expression of Ri T-DNA genes rather than to persisting Agrobacteria in the plant tissues. No major difference was observed in size and shape between nodules developed on transformed and untransformed roots. Acetylene-reduction activity exhibited by transformed and untransformed 4-week-old nodules was comparable (45)lmol C2 HJg dry weight/nodule/h).

In: Pinyopusarerk K, Turnbull JW, Midgley SJ (eds) Recent casuarina research and development. Forestry and Forest Products, CSIRO. Australia, pp 104-108 Pawlowski K, Bisseling T (1996) Rhizobial and actinorhizal symbioses: what are the shared features? Plant Cell 6:1899-1913 Phelep M, Petit A, Martin L, Duhoux E, Tempe J (1991) Transformation and regeneration of a nitmgen-fixing tree, Allocasuarina verticillata Lam. BiofTechnology 9:461-466 Rogers SO, Bendich AJ (1985) Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues.

Tumefaciens were optimized on Casuarina gLauca (Le et at. 1996). The influence of the following parameters was studied: the selection marker and its concentration (kanamycin or hygrornycin from 5 to 200mgtl). the strain of A. tumefaciens (C58C1(GV2260; pBIN19GUSINT), LBA 4404(pAlM04; pBIN19GUSINT), and LBA4404(pAL4404; pT0K233)), the explant (hypocotyls, cotyledons, or epicotyls), the addition of acetosyringone (0 to lOOIlM) during cocultivation. the duration of cocultivation (one to 7 days), the age of the explants, the pH of cocultivation (5 to 6), and a pretreatment of the explants by naked microprojectiles.

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A Natural History of Trees: of Eastern and Central North America by Donald Peattie, Paul Landacre, Robert Finch


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