By M. Hamon
ISBN-10: 2851802380
ISBN-13: 9782851802385
Read or Download Aïkido. Une tradition, un art, un sport French PDF
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Suppressor colonies will appear mainly at the border between those areas getting lethal doses of UV-light and those in the shadow of the lid. D) Analysis of the Plasmids As re-isolation of plasmids from yeast cells yields only very low amounts of DNA, which are insufficient for further analysis, re-isolated plasmid DNA must first be transformed into E. coli cells and isolated in larger quantities from the bacterial cells. All of the common yeast plasmids are yeast-E. coli shuttle plasmids. Re-isolation of plasmids from yeast cells can be performed under the same standard protocols as used for E.
Cerevisiae, such as in the expression of foreign proteins for research, industrial, or medical use or as a scientific model organism. Advances in yeast genetics and molecular biology techniques have made S. cerevisiae an excellent choice for the characterization of general metabolic and regulatory processes. A unique property of S. cerevisiae is its high specialization for converting certain sugars to ethanol and carbon dioxide. However, this yeast cannot use a very wide range of sugars. The preferred carbon sources of S.
Cerevisiae hxt-null strain, which is unable to take up and to grow on glucose as the sole carbon source. First, uptake of the gene library plasmids was selected on medium without uracil (as the plasmids carried the URA3-selectable marker gene) and with maltose as a permissive carbon source. The transformants were replica plated onto selective medium with 2% glucose. After some days of incubation at 30°C, several colonies that could grow on glucose appeared. The plasmids were reisolated out of the transformants, further characterized, and sequenced.
Aïkido. Une tradition, un art, un sport French by M. Hamon
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