By Mitsuo Satoh, Shigeru Iida (auth.), Sanetaka Shirahata, Koji Ikura, Masaya Nagao, Akira Ichikawa, Kiichiro Teruya (eds.)
Animal telephone expertise is a becoming self-discipline of telephone biology which goals not just to appreciate buildings, features and behaviors of differentiated animal cells, but additionally to check their skills for use for commercial and clinical reasons. The target of animal phone know-how comprises the clonal enlargement of differentiated cells, the optimization in their tradition stipulations, modulation in their skill to provide proteins of clinical and pharmaceutical importantance, and the applying of animal cells to gene remedy, man made organs and the construction of practical meals. This quantity offers the readers a whole evaluate of the current cutting-edge and should be worthy for these operating in both educational environments or within the biotechnology and pharmaceutical sectors, relatively mobile biologists, biochemists, molecular biologists, immunologists, biochemical engineers and all different disciplines with regards to animal mobilephone culture.
Read Online or Download Animal Cell Technology: Basic & Applied Aspects: Proceedings of the 19th Annual Meeting of the Japanese Association for Animal Cell Technology (JAACT), Kyoto, Japan, September 25-28, 2006 PDF
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Additional resources for Animal Cell Technology: Basic & Applied Aspects: Proceedings of the 19th Annual Meeting of the Japanese Association for Animal Cell Technology (JAACT), Kyoto, Japan, September 25-28, 2006
King, R. and Wells, J. R. (1992) Novel Recombinant Fusion Protein Analogues of Insulin-Like Growth Factor (IGF)-I Indicate the Relative Importance of IGF-Binding Protein and Receptor Binding for Enhanced Biological Potency. Journal of Molecular Endocrinology 8 (3): 213–223. 5. , Negrete-Virgen, J. , Lyddiatt, A. and Al-Rubeai, M. (2002) Rapid Titration of Adenoviral Infectivity by Flow Cytometry in Batch Culture of Infected HEK 293 Cells. Cytotechnology 38: 87–97. Simple and Efficient Establishment of Recombinant Protein Hyper-Producing Cells by Using RAS-Amplified CHO Cell Line Tsukasa Fujiki, Toshiki Matsuo, Makiko Yamashita, Yoshinori Katakura, Shin-Ei Matsumoto, Kiichiro Teruya, and Sanetaka Shirahata Abstract Chinese hamster ovary (CHO) cells is a widely used host cell line and can massproduce recombinant proteins by using various amplifiable selectable marker gene and selection drugs.
48-113-2, Yonozu, Mikuni-Cho, Sakai, Fukui, 913–0038 K. Ikura et al. V. 2009 41 42 T. Toyosawa et al. important for stable cell culture. However, freeze–thawing gives rise to cell damage and often leads to a high level of cell death. As a result, cryoprotectants are added to the cryopreservative solution. Currently, fetal bovine serum (FBS) is extensively used as such a cryoprotectant, although FBS carries the risk of infection with abnormal prion proteins and viruses, and therefore should be avoided.
Then, we demonstrated CHO cell lines transfected with ras-oncogene Target gene (AE6F4) vector c-Ha-ras, dhfr gene vector Marker protein (CD4) vector Co-transfection into CHO-dhfr(-) and CHO-hp1 FACS cell sorting MACS (MA gnetic Cell Sorting) MTX (Gene amplification) Limiting dilution cloning Single cell sorting 1~2 weeks 1~2 days Low productive and weak clones Highly productive clones and rapid cloning CHO-dhfr(-) 0 clones/1000 seeded wells CHO-hp1 188 clones/1000 seeded wells Fig. 1 The method of the establishment of highly productive cell line 28 T.
Animal Cell Technology: Basic & Applied Aspects: Proceedings of the 19th Annual Meeting of the Japanese Association for Animal Cell Technology (JAACT), Kyoto, Japan, September 25-28, 2006 by Mitsuo Satoh, Shigeru Iida (auth.), Sanetaka Shirahata, Koji Ikura, Masaya Nagao, Akira Ichikawa, Kiichiro Teruya (eds.)